Michal Greguš(1*), Pavol Ďurč(2), Júlia Lačná(3), František Foret(4), Petr Kubáň(5)

(1)  Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00 Brno, CZECH REPUBLIC; Department of Chemistry, Masaryk University, Kotlářská 267/2, 611 37 Brno, CZECH REPUBLIC
(2)  Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00 Brno, CZECH REPUBLIC; Department of Chemistry, Masaryk University, Kotlářská 267/2, 611 37 Brno, CZECH REPUBLIC
(3)  Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00 Brno, CZECH REPUBLIC; Department of Chemistry, Masaryk University, Kotlářská 267/2, 611 37 Brno, CZECH REPUBLIC
(4)  Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00 Brno, CZECH REPUBLIC; Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, v.v.i., Veveří 97, 602 00 Brno, CZECH REPUBLIC
(5)  Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00 Brno, CZECH REPUBLIC; Department of Chemistry, Masaryk University, Kotlářská 267/2, 611 37 Brno, CZECH REPUBLIC; Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, v.v.i., Veveří 97, 602 00 Brno, CZECH REPUBLIC
(*) Corresponding Author

Study of Various Parameters that Influence the Content of Exhaled breath Condensate Used in the Diagnosis of Gastroesophageal Reflux Disease


Abstract



In this work, various parameters that influence the ionic content and pH of exhaled breath condensate in terms of the noninvasive diagnosis of gastroesophageal reflux disease were studied. Exhaled breath condensate samples were collected using a miniature and inexpensive sampling device. Capillary electrophoresis with contactless conductometric detection was used to monitor the ionic content of exhaled breath condensate. Background electrolyte composed of 20 mM of 2-(N-Morpholino)ethanesulfonic acid, 20 mM of L-Histidine, 2 mM of 18-Crown-6 and 30 µM of cetyltrimethylammonium bromide facilitated the rapid separation of anions and cations, both in less than 2 minutes. The possibility of contamination of the exhaled breath condensate by saliva is discussed in detail. The day-to-day repeatability (n=5) of the ionic content and pH of the exhaled breath condensate was studied and was satisfactory, reflecting mainly the physiological variability.

Keywords


capillary electrophoresis; exhaled breath condensate; ionic analysis; pH; gastroesophageal reflux disease

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