Peter Troška(1*), Anna Mandžáková(2), Jasna Hradski(3), Juraj Ševčík(4), Marián Masár(5)

(1)  Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina CH-2, Ilkovičova 6, SK-84215 Bratislava, SLOVAK REPUBLIC
(2)  Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina CH-2, Ilkovičova 6, SK-84215 Bratislava, SLOVAK REPUBLIC
(3)  Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina CH-2, Ilkovičova 6, SK-84215 Bratislava, SLOVAK REPUBLIC
(4)  Department of Analytical Chemistry, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, CZ-77146 Olomouc, CZECH REPUBLIC
(5)  Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina CH-2, Ilkovičova 6, SK-84215 Bratislava, SLOVAK REPUBLIC
(*) Corresponding Author

Determination of Homocysteine in Urine and Saliva by Microchip Electrophoresis


Abstract



An online combination of isotachophoresis (ITP) and capillary zone electrophoresis (CZE) was employed on a microchip with a column-coupling technology for the determination of homocysteine in various body fluids. ITP with its high concentration ability was used as a sample pretreatment and injection technique for CZE, which facilitated the rapid and sensitive determination of homocysteine. The resolution of the analyte from other constituents present in real complex samples was enhanced by discrete spacers, which were added to the injected sample. A solid-phase microextraction (SPME) pretreatment technique based on silver- and barium-form resins was used prior to the ITP-CZE analysis to remove high concentrations of chloride and sulfate naturally present in the analyzed samples. The combination of the micropretreatment and microelectrophoresis techniques facilitated the determination of trace concentrations of homocysteine in samples of urine and saliva.

Keywords


homocysteine; body fluids; microchip electrophoresis; solid-phase microextraction

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